CULTURE MEDIA - BRAIN HEART INFUSION

It is a mixture of calf brain infusion and beef heart infusion. Each 17.5 grams of the powder contains infusions from 200 grams calf brain and 250 grams beef heart.

Quality Control
PHYSICAL DATA
Appearance	Light brown amorphous powder
Odour	Characteristic
pH (2% solution)	6.0 to 7.0
Solubility	Freely soluble in water forming sparklingly clear soln
Loss on Drying	Not more than 5.0%
CHEMICAL DATA
Total Nitrogen (Kjaldal method) content TN	Not less than 13.5%
Amino Nitrogen AN	Not less than 3.5 %
Degree of Hydrolysis  DH = (AN/TN) X 100	Not less than 25%
Ash	Not more than 10%
Proteose Test (As per USP)	Passes
Coagulable protein  (As per USP)	Passes
BACTERIOLOGICAL DATA
Total Plate Count	Not more than 5000CFU/gm
E.coli/10 gm	Absent
Salmonella/10gm	Absent