CULTURE MEDIA - TRYPTONE (Enzymatic digest of Casein)
It is a pancreatic digest of milk protein casein. It is a very rich source of amino acid nitrogen. It has a high tryptophan content and therefore used in media for testing indole reaction, sterility testing media and fermentation process.
It is a pancreatic digest of milk protein casein. It is a very rich source of amino acid nitrogen. It has a high tryptophan content and therefore used in media for testing indole reaction, sterility testing media and fermentation process.
| Quality Control | |
|---|---|
| PHYSICAL DATA | |
| Appearance | Light cream amorphous powder |
| Odour | Characteristic proteinous odour |
| pH (2% solution) | 6.0 to 7.0 |
| Solubility | Freely soluble in water forming sparklingly clear soln |
| Loss on Drying | Not more than 5.0% |
| CHEMICAL DATA | |
| Total Nitrogen (Kjaldal method) content TN | Not less than 13% |
| Amino Nitrogen AN | 4.5 % |
| Total Protein content (kjaldal factor- 6.25) |
Not less than 80% |
| Degree of Hydrolysis DH = (AN/TN) X 100 |
Not less than 30% |
| Ash | Not more than 10% |
| Proteose Test (As per USP) | Passes |
| Coagulable protein (As per USP) |
Passes |
| BACTERIOLOGICAL DATA | |
| Total Plate Count | Not more than 5000CFU/gm |
| E.coli/10 gm | Absent |
| Salmonella/10gm | Absent |