CULTURE MEDIA - TRYPTOSE
Tryptose is a mixture of enzymically digested protein. It is designed to promote luxuriant growth of highly fastidious microorganisms. Even though enzymically digested, it can be successfully employed in place of meat infusion to meet the nutritional requirements of fastidious microorganisms. It is a light yellow coloured powder that forms crystal clear aqueous solution. It is used in following media. Tryptose Media – for cultivation of fastidious microorganisms and for preparing Blood Agars. Since the blood cells are maintained in an excellent state in Tryptose Media, haemolytic reactions can be easily demonstrated. Vaccine preparation media – for rapid and luxuriant growth as desired for large scale manufacturing of vaccines and toxins.
Tryptose is a mixture of enzymically digested protein. It is designed to promote luxuriant growth of highly fastidious microorganisms. Even though enzymically digested, it can be successfully employed in place of meat infusion to meet the nutritional requirements of fastidious microorganisms. It is a light yellow coloured powder that forms crystal clear aqueous solution. It is used in following media. Tryptose Media – for cultivation of fastidious microorganisms and for preparing Blood Agars. Since the blood cells are maintained in an excellent state in Tryptose Media, haemolytic reactions can be easily demonstrated. Vaccine preparation media – for rapid and luxuriant growth as desired for large scale manufacturing of vaccines and toxins.
| Quality Control | |
|---|---|
| PHYSICAL DATA | |
| Appearance | Light yellow to yellow homogeneous free flowing powder having characteristic odour but not putrescent |
| Solubility | Freely Soluble in distilled purified water insoluble in alcohol. |
| Clarity | 1%w/v aqueous solution is clear and neutral without any haziness after autoclaving at 15 lbs pressure (121°C) for 15 minutes |
| Reaction | Reaction of 2%w/v aqueous solution at 25°C pH:6.7±0.5 |
| Microbial Load | Total aerobic microbial count (cfu/gm): By plate method when incubated at 35-37°C for 24-48 hours Bacterial count: <=2000.00 (cfu/gm) Total yeast and mold count (cfu/gm): By plate method, when incubated at 20-25°C for not less than 5 days Total yeast and mold count: <=100.00 (cfu/gm |
| Indole test | Tryptophan content: Passes |
| CHEMICAL DATA | |
| Total Nitrogen | >=12.50% |
| Amino Nitrogen | >=4.00% |
| Sodium Chloride | <=5.00% |
| Loss on drying | <=5.00% |
| Residue on ignition | <=12.00% |