CULTURE MEDIA - YEAST EXTRACT

Yeast extract is water soluble, peptone like derivative of yeast cells (Saccharomyces). It is manufactured under controlled conditions to retain its vitamin content and other nutritive values such as free amino acids. Yeast extract is a substrate in media formulation not only supplies vitamins but also proteins, carbohydrates and some micronutrients.

Quality Control
PHYSICAL DATA
Appearance	Dine pale yellow powder
Odour	Characteristic
pH (2% solution)	6.0 to 7.0
Solubility	Freely soluble in water forming sparklingly clear soln
Loss on Drying	Not more than 5.0%
CHEMICAL DATA
Total Nitrogen (Kjaldal method) content TN	Not less than 14%
Amino Nitrogen AN	Not less than 5.0%
Total Protein content  (kjaldal factor- 6.25)	Not less than 80%
Degree of Hydrolysis  DH = (AN/TN) X 100	Not less than 45%
Ash	Not more than 10%
Proteose Test (As per USP)	Passes
Coagulable protein  (As per USP)	Passes
BACTERIOLOGICAL DATA
Total Plate Count	Not more than 5000CFU/gm
E.coli/10 gm	Absent
Salmonella/10gm	Absent